optek AF46 dual wavelength uv absorption analyzer mounted on a biotech tri-clamp sensor body

AF46 UV Analyzer

Dual Wavelength UV Absorption Analyzer

The AF46 is a dual wavelength, high precision UV light absorption analyzer designed for inline operation to provide accurate concentration measurements with remarkable repeatability, linearity and resolution. Wavelength and optical path length can be individually configured to specific requirements for the highest accuracy.

Used For

UV range:
- 0 - 0.05 to 2 CU (concentration units), dependent on filter/OPL used
UV absorption control and UV Absorption control
- in Chromatography: Pre / Post column monitoring (OD) for optimized pooling
- of concentrations in permeate or retentate
- in TFF / Ultrafiltration
- of organic load in water / condensate
- of the concentration of aromatics (Caprolactam, Aniline, Phenol, etc.)
- as TOC, COD, BOD trend analysis
Disinfection control treatment of water

Features

Benefits

UV analyzer for dual wavelength absorbance measurement with discrete lamp references

Simultaneous precise UV measurements at 2 wavelengths with compensation of lamp effects

Various UV wavelengths available

Optimized solution for specific applications requirements

Prepared for use of calibration filters

Easy validation by user with NIST traceable calibrated filters

Long lasting light source

reliable process monitoring with reduced maintenance

High temperature version available

Suitable for SIP process

The AF46 is a dual wavelength UV analyzer ideally suited to monitor protein concentrations (e.g., monoclonal antibodies) in downstream biotechnology applications such as chromatography or filtration. Using two different wavelengths the sensor is capable of detecting very low protein concentrations for accurate pooling and at the same time is able to monitor the whole elution peak with very high optical density (OD) using a second wavelength.

Besides protein measurements the sensor is often used to monitor nucleic acid contaminations of protein solutions. Due to the modularity different optical path lengths (OPL) and wavelengths can be combined to measure any product absorbing in the UV range.

AF46
Schematic	Sensor Body Windows Beam splitter Filter A Measurement detector A Filter B Measurement detector B Reference detector A Reference detector B Lamp module (mercury)
Measurement
Measurement principle	2- Channel Absorption of light
Measurement wavelength(s)	254-13 / 280-13, 254-13 / 313-13, 280-09 / 300-05, 280-09 / 300-13, 280-09 / 313-13, 280-13 / 300-13, 280-13 / 313-13, 290-13 / 313-13 nm, ... others on request
Detector(s)	2 silicon photodiodes (hermetically sealed)
Reference detector(s)	2 silicon photodiodes (hermetically sealed)
Measuring Range	any measuring range between 0 - 0.05 to 2 CU (dependent on filter used / OPL) (contact product specialist for application specific ranges)
Optical path length	1 - 160 mm
Calibration	CU (concentration units) application specific calibration
Light source	low pressure mercury lamp typical life span: 1 to 2 years (8,000 to 16,000 hours)
Resolution	< ± 0.05 % of respective measuring range
Repeatability	< ± 0.5 % of respective measuring range
Linearity	< ± 1 % of respective measuring range (specific to application)
Protection	all optical parts have an IP rating of IP65 or higher
Sensor Body
Material	Stainless steel 1.4435 (SS 316L), 1.4539 (904 L), 1.4571 (SS 316Ti), 1.4462 (318 LN), Titanium 3.7035 (Grade 2), Hastelloy 2.4602 (C22), Plastic TFM™ 4215, ... others on request
Line size	1/8 in. to 6 in. (DN 6 to DN 150), ... others on request
Process connection	Flanges (ASME, DIN, EN, JIS), Clamps (TC, ISO, DIN), Female Threads (NPT, DIN), Sanitary Threads (DIN 11851), Tube Ends (DIN, ISO, OD),DIN 11864-1/-2/-3 (DIN, ISO, OD), Varivent (DIN, IPS, OD), ... others on request
Process pressure	0 - 100 bar (0 - 1450 psi) - higher on request depending on process connection, materials and design
Windows	2-Sapphire, 3-Sapphire Biotech (do not use Pyrex®)
Window gaskets	Viton® (FDA), EPDM / Kalrez® 6230 (FDA / USP Class VI), Kalrez® 4079, ... others on request (do not use Silicone)
Temperature Ratings
Process temperature	permanent: 0 - 70 °C ( 32 - 158 °F) peak 15 min/day: 0 - 135 °C ( 32 - 275 °F) peak 30 min/day: 0 - 120 °C ( 32 - 248 °F)
Process temperature OPTION HT	permanent: -30 - 120 °C (-22 - 248 °F) peak 15 min/day: -30 - 150 °C (-22 - 302 °F) peak 30 min/day: -30 - 140 °C (-22 - 284 °F)
Process temperature OPTION EX	permanent: -30 - 70 °C (-22 - 158 °F) peak 15 min/day: -30 - 135 °C (-22 - 275 °F) peak 30 min/day: -30 - 120 °C (-22 - 248 °F)
Process temperature OPTION EX-HT	permanent: -30 - 120 °C (-22 - 248 °F) peak 15 min/day: -30 - 150 °C (-22 - 302 °F) peak 30 min/day: -30 - 140 °C (-22 - 284 °F)
Ambient temperature	permanent: 0 - 40 °C ( 32 - 104 °F) operation: -30 - 40 °C (-22 - 104 °F) with options HT / EX / EX-HT transport: -20 - 70 °C ( -4 - 158 °F)
Explosion Proof
Ex-proof	none
Ex-proof OPTION EX (EN-D)	Sensor assembly in ex-proof version accor. ATEX (EN-D)
Ex-proof OPTION EX (FM-D)	Sensor assembly in ex-proof version accor. FM (FM-D)
Calibration
Calibration adapter VB	Filter adapter FH03 for calibration filter used for sensor verification
Pressure and temperature ratings specified herein may be subject to limitations - see instruction manual. The appropriate choice of material for all wetted parts is the sole responsibility of the user. Data given are subject to changes without prior notice.