Chromatography

Pre-Column Chromatography Monitoring

An inline ACF60 conductivity sensor, which includes a temperature sensor, and the PF12 pH sensor give real-time measurements of conductivity, temperature and pH.

Post-Column Chromatography Monitoring

Accurate, reliable and repeatable post-column measurements are necessary during purification to ensure accurate pooling to maximize yields and protein/DNA fraction purity.

Mounted directly inline in the column outlet stream, an optek single wavelength AF45 or dual wavelength AF46 UV sensor can improve the efficiency of the separation process. This ensures product purity and greatly reduces off-line testing and manual analysis.

optek AF46-VB Dual Channel UV-Absorption Sensor with Calibration Adapter

A NIST-traceable filter package allows users to calibrate the sensors inline in a quick and repeatable manner.

The six-electrode, four-pole conductivity sensor ACF60 and a PF12 pH sensor monitor the process while being mounted in a single optimized flow cell with low hold-up volume. A wide measuring range of the conductivity sensor allows monitoring transitions between buffers and control of the cleaning process, while a PF12 sensor is used for adjustment of the gel packing by means of the pH value. Additionally, equilibration methods are monitored and column integrity is ensured.

Dual Wavelength

optek ACF60 Conductivity Sensor and PF12 pH Electrode Adapter mounted on a Biotech Armature

The primary wavelength measures concentration of protein at e.g. 280 nm for pooling purposes. Using the secondary wavelength measuring e.g. at 300 nm simultaneously, this signal is used to achieve readings even at the highest concentration ranges.

Dual-wavelengths provide precise resolution at low OD (Optical Density) to start or stop collection of the fraction using 280 nm. A high-range secondary wavelength is used to monitor elution at the highest OD ranges.

Measurements at wavelengths 254/280 nm assess purity level and nucleic acid (DNA/RNA) contamination of protein. Due to the modularity of optek inline sensors, different optical path lengths (OPL) are possible. Depending on concentration range required, the OPL and the wavelength combination are specified to achieve the highest repeatability and linearity.

C8000 - 8 measurements taken from 5 sensors with 1 high precision converter

In a chromatographic separation skid the following measurements are required:

PRE-Column:

combined in one sensor body for low hold-up volume

• conductivity (optek ACF60)
• temperature (optek ACF60)
• pH (optek PF12)

POST-Column:

combined in two sensor bodies for low hold-up volume

• dual channel UV (optek AF46)
• conductivity (optek ACF60)
• temperature (optek ACF60)
• pH (optek PF12)

optek C8000 Universal Converter