optek UV sensors can be optimized for both high OD and low end resolution for monitoring real time protein concentration. optek AF45 or AF46 sensors have proven time and again to provide accurate measurements, better repeatability, and linear response. Inline photometric analysis can save time and money by eliminating human error from column fractionation and offline spectrophotometer testing.
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Applicable to nearly any type of non-adsorptive and adsorptive chromatography such as Ion Exchange Chromatography (IEC), gel-filtration or Size-Exclusion Chromatography (SEC), Affinity chromatography, and Hydrophobic Interaction Chromatography (HIC). optek inline photometric and electro-chemical sensors are designed specifically for real-time measurement and control.
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Chromatographic Separation Benefits |
- Improved Product Quality
- Improved Yields
- Reduced Effluent Loading
- Improved Dehydration and Salt Removal
- Improved Column Efficiency
- Reduced Costs
- Real-time Process Measurement and Control
- Immediate Detection of Upsets and Imbalance
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Inline photometry is a valuable tool to improve chromatographic separations, generating consistent, repeatable data to allow more aggressive pooling criteria for maximizing yields and improving quality.
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| Pre-Column Process Measurements - Bubble Detection |
An optek single or dual wavelength UV absorption sensor installed in the column feed stream provides immediate and extremely reliable detection of process disturbances to avoid damage to column media.
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Measuring at a specific region of the spectrum, optek AF45/46 or TF16 inline sensors can differentiate between phase changes and immediately alert the process operator should gas bubbles be present or if an empty pipe conditions occurs in both aqueous and non-aqueous solutions.
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| Low Volume Inline pH & Conductivity |
It is important to measure pH and conductivity of elution buffers and other solutions upstream of the column. For these analyses, optek offers a unique line of pH and conductivity sensors designed for bioprocess applications.
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Uniquely adapted to optek inline, flow through sensor bodies, the ultra-sanitary PF12 pH sensor and CF60 conductivity electrodes are ideal for accurate and reliable electro-chemical measurements with extremely low hold-up volumes and excellent resistance for CIP and sterilization.
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| Post-Column Chromatography Monitoring |
During purification, accurate, reliable and repeatable post-column measurement is necessary to ensure accurate pooling to maximize yields and protein fraction purity.
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Mounted inline directly in the column outlet stream, an optek AF45 or AF46 UV sensor can improve the efficiency of the separation process and greatly reduce off-line testing and manual analyses.
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| Chromatographic Separation Summary |
When chromatography is used for filtering and purification, accurate and repeatable data is essential, to allow more aggressive pooling criteria for maximizing yields and improving quality. Because of the importance of this process step, biopharmaceutical manufacturers need precise and reliable in-line measurements to gain the highest level of separation performance and maximize fraction yields.
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NIST-traceable inline validation filters provide fast and repeatable measurement verification without breaching sterility. Superior Sapphire optics and an ultra-sanitary stainless steel body allow optek sensors to withstand tough process environments and CIP/SIP.
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optek pH and conductivity measurements complete the picture by adding accurate and reliable electro-chemical measurements to assist in your precise control of the chromatography process.
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